As shown in Fig. 4, in contrast to its stability in a solid state, creatine is not stable in aqueous solution due to an intramolecular cyclization (Howard and Harris 1999). The rate of creatine degradation in solution is not dependent on its concentration, but on pH. Generally, the lower the pH and higher the temperature, the faster is the degradation. This solid-state and degradation properties have been thoroughly investigated as early as the 1920s (Edgar and Shiver 1925; Cannon et al. 1990) and more recently by Dash et al. (2002), as well as Harris et al. (Howard and Harris 1999). These researchers found that whereas creatine was relatively stable in solution at neutral pH (7.5 or 6.5), a lowering of pH resulted in an increased rate of degradation and after only 3 days of storage at 25°C creatine degraded significantly: 4% at pH 5.5; 12% at pH 4.5; and 21% at pH 3.5 (see Fig. 5). Similarly, Ganguly et al. (2003) reported that creatine monohydrate stored at room temperature degraded into creatinine within several days but that refrigerating creatine monohydrate in solution slowed degradation. The rapid degradation of creatine in solution precludes the manufacture of shelf-stable standard acidic beverages containing efficacious amounts of the ingredient. If creatine is not consumed immediately after it has been dissolved in water, it should be stored at a low temperature to retard the degradation.