Why not?
Isnt Acetic Acid a mixure of water & white vinigar.
found this on another board
Items Needed:
***8226; Distilled white vinegar (grocery store)
***8226; Distilled water (grocery store)
***8226; 0.2-0.22um sterile Whatman
syringe filter
***8226; 10mL
syringe with a luer lock tip
***8226; ~20-22 gauge
needles (just the
needles)
***8226; Sterile glass
vial (10-20mL)
***8226; Alcohol prep pads ***8211; sterile kind (70% isopropyl alcohol)
Quick Guide:
1. Swab the top of your sterile
vial with alcohol prep pad (70% isopropyl alcohol)
2. Mix 7.5mL distilled water with 1.0mL vinegar
3. Add Whatman
syringe filter
4. Add sterile ~20ga. needle to end of Whatman filter
5. Inject the 8.5mL of solution into the sterile
vial
6. You now have sterile 0.6% acetic acid
Detailed Directions:
1. Wash you hands thoroughly
2. Optional: wear alcohol treated exam gloves (rub your gloved hands together with 70% isopropyl alcohol on them until dry)
3. Using a sterile alcohol prep pad, swab the top of your sterile glass
vial (into which the acetic acid solution will be held in)
4. Using the 10mL
syringe with a ~20ga. needle on the end, draw up 7.5mL distilled water
5. Using the same
syringe, now draw up 1mL vinegar
6. Remove needle from the
syringe and discard
7. Attach 0.2-0.22um Whatman sterile
syringe filter (do not touch the free end that will have a needle on it)
8. Put a new, sterile needle (~20 gauge) onto the free end of the Whatman filter (do not touch needle)
a. Do not use the same needle on the Whatman that was used to originally draw up the unsterile vinegar and distilled water.
9. Put a ~20 gauge sterile needle into the top of your sterile glass
vial to act as a vent
10. Inject the acetic acid solution into the
vial
11. You are now done and should have sterile 0.6% acetic acid
Notes:
1. These items MUST be sterile: 20-22ga.
needles, whatman filter, glass
vial
2. Whatman filter: These small, sterile filters are used to filter the acetic acid solution so it is sterile. It does not matter that the liquid in your
syringe (distilled water & vinegar) is not sterile, nor does it matter that the
syringe itself is not sterile. Once the liquid goes through the filter it is STERILE. Thus, everything after the filter must be sterile!
3. You will most likely use 1mL (milliliter) of 0.6% AA to reconstitute your
igf-1. Thus, you should make at least 1.5mL. In reality, it***8217;s just as easy to make 8.5mL as I have stated in the above directions. You will have plenty for use later then.
4. Do NOT reuse the Whatman filter nor any
needles! Discard immediately.